数字PCR平台与单细胞单分子检测

数字PCR作为第三代PCR技术,主要是在扩增单分子的基础上对核酸拷贝数进行绝对性定量的样品检测技术,具有非常高的灵敏度.近年来,随着科学技术的进步和医疗卫生技术水平的改善提高,微流控技术作为一种先进的技术,促使着数字PCR平台的产生.但是现有的数字PCR平台类型主要是芯片型和液滴型,不仅操作费用昂贵,且需要进行外接的相关配套控制系统以及管路众多,这便在很大程度上严重制约了我国生命科学领域中对于数字PCR技术的广泛应用.

Digital PCR Platform and Single Cell Single Molecule Detection

Digital PCR as the third generation of PCR technology, mainly in the amplification of single molecules on the basis of nucleic acid copy number absolute quantitative sample detection technology, with very high sensitivity. In recent years, with the advancement of science and technology and improvement of medical and health technology, microfluidic technology as an advanced technology, promote the generation of digital PCR platform. However, the existing digital PCR platform types are mainly chip type and droplet type, not only expensive to operate, and the need for external control system and the associated pipeline, which in large part severely restricted the field of life sciences For the wide application of digital PCR technology.