HPLC测定六神曲中青蒿素、芦丁和槲皮素含量

本研究建立了高效液相色谱法测定六神曲中青蒿素、芦丁和槲皮素成分含量的方法。采用AcchromXAquaC18色谱柱（4.6mm×250 mm,5μm）。测定青蒿素含量时,用柱前衍生方法,以乙腈-水（60∶40）为流动相;流速0.8 mL/min,紫外检测波长为260 nm,柱温35℃。测定芦丁和槲皮素含量时,以甲醇-0.4%磷酸（50∶50）为流动相;紫外检测波长分别为360 nm（芦丁）和280 nm（槲皮素）;流速0.8 mL/min;柱温30℃。结果青蒿素、芦丁和槲皮素可以达到较好的分离。在选定质量浓度范围内线性关系良好（青蒿素R2=0.9991,芦丁R2=0.9994,槲皮素R2=0.9995）,平均回收率分别为98.6%,101.4%和97.2%,相对标准偏差（RSD）分别为1.65%,0.91%和1.46%（n=5）。该方法简便准确,重复性和稳定性较好,为六神曲的质量评价和控制提供了科学依据。

Determination of artemisinin,rutin and quercetin in Massa Medicata Fermentata by HPLC

HPLC method was described for determining the contents of artemisinin, rutin and quercetin in Massa Medicata Fermentata（MMF）. Chromatography separation was performed on HPLC system with Acchrom XAqua C18（4.6 mm×250 mm, 5 μm） column. Artemisinin was determined with the method of pre-column reaction and isocratic elution with mobile phase acetonitrile/water（60∶40）, the flow rate was 0.8 ml/min, and UV detection wavelength and temperature was 260 nm and 35 ℃, respectively. Rutin and quercetin were determined under the condition of mobile phase methanol/0.4% phosphoric acid（50∶50）, flow rate 0.8 ml/min, temperature 30 ℃, detection wavelength 360 nm for rutin and 280 nm for quercetin,respectively. The results indicated that the separation of artemisinin, rutin and quercetin was good. A good linearity was observed in the selected concentration range（artemisinin R2=0.999 1, rutin R2=0.999 4 and quercetin R2=0.999 5）. The average recoveries of the three compounds were 98.6%（RSD 1.65%）, 101.4%（RSD 0.91%） and 97.2%（RSD 1.46%）（n=5）, respectively. The method was simple with good accuracy and reproductivity and it can be applied for quality evaluation and control for Massa Medicata Fermentata.