产酸性甘露聚糖酶菌株的筛选、鉴定及酶活性研究 |
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引用本文: | 张居作,李志波,徐君飞.产酸性甘露聚糖酶菌株的筛选、鉴定及酶活性研究[J].中国酿造,2014(11):63-66. |
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作者姓名: | 张居作 李志波 徐君飞 |
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作者单位: | 怀化学院生命科学系民族药用植物资源研究与利用湖南省重点实验室;湖南农业大学动物医学院; |
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基金项目: | 湖南省科技厅项目(2013NK4109);湖南省重点学科建设项目资助(2011-42);民族药用植物活性成分高效利用湖南省高校创新团队经费资助(2010-53) |
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摘 要: | 采用酸性魔芋精粉平板,从土壤中分离到两株产酸性甘露聚糖酶菌株,命名为LZ11、LZ12;分别对菌株LZ11、LZ12在生长过程中分泌酸性甘露聚糖酶的活力进行跟踪研究后发现,这两株菌株的对数生长期皆为2~12 h,发酵8 h后,酶活力呈明显上升趋势,14 h达到峰值,分别为23.15 U ,19.09 U ;选择酶活力稍高的菌株LZ11进行鉴定,根据生理生化试验结果,初步鉴定为芽孢杆菌属(B acillussp.)。
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关 键 词: | 甘露聚糖酶 筛选 鉴定 酶活性 |
Screening and identification of acidic mannase-producing strain and its enzymatic activity |
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Affiliation: | ZHANG Juzuo, LI Zhibo, XU Junfei (1.Key Laboratory of Hunan Province for Study and UtlTization of Ethnic Mech'cinal Plant Resources, Department of Life Science, Huaihua University, Huaihua 418008, China; 2.Faculty of Veterinary, Hunan Agricultural University, Changsha 410128, China) |
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Abstract: | Tw o acidic m annase-producing strains w ere isolated from soilby acidic konjac pow derplate,and w ere nam ed LZ11 and LZ12.The m an-nase activitiessecreted by strain LZ11 and LZ12 w ere tracked and theirgrow th curve show ed thatthe logarithm ic phase w asboth at2-12 h.A fter8 h ferm entation,the enzym e activity obviously increased,and the peak w asatthe 14 h.A tthattim e,the enzym e activitiesofstrain LZ11 and LZ12 w ere 23.15 U and 19.09 U ,respectively.The strain LZ11 w ith higherenzym e activity w as selected and identified as B acillussp.according to the physio-logicaland biochem icaltestresults. |
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Keywords: | m annase screening identification enzym atic activity |
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