| 大围山森林土壤中高产纤维素酶菌株的筛选及鉴定 |
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| 引用本文： | 李悦,李世俊,薛桥丽,胡永金.大围山森林土壤中高产纤维素酶菌株的筛选及鉴定[J].中国酿造,2014(6):128-131. |
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| 作者姓名： | 李悦 李世俊 薛桥丽 胡永金 |
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| 作者单位： | 云南农业大学食品科学技术学院;云南农业大学图书馆; |
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| 基金项目： | 国家自然科学基金项目(30960308);云南省自然科学基金项目(2008ZC057M) |
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| 摘 要： | 通过初筛（刚果红纤维素水解试验和滤纸条分解试验）和复筛（利用3,5-二硝基水杨酸法测定内切纤维素酶活,滤纸酶活和β-葡萄糖苷酶活）,从大围山原始森林土壤中筛选高产纤维素酶菌株,获得4株具有高产纤维素酶活性的菌株。其中一株真菌16-7分解纤维素能力最强且酶活稳定,其内切羧甲基纤维素酶活为265.76U/mL,滤纸酶活为86.44 U/mL,β-葡萄糖苷酶活为39.16 U/mL;对真菌16-7分别进行形态学观察、分子生物学鉴定,初步确认为小刺青霉（Penicilliumspinulosum）。
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| 关 键 词： | 土壤 高产纤维素酶菌 筛选 酶活 |
Screening and identification of high cellulase-producing strains from Dawei Mountain forest soil |
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| Affiliation: | LI Yue, LI Shijun, XUE Qiaoli, HU Yongjin (1.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2.Library, Yurman Agricultural University, Kunming 650201, China) |
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| Abstract: | High cellulase-producing strain from Dawei forest soil was screened using first screening（Congo red cellulase hydrolysis test and filter decomposition test） and secondary screening（determination of CMCase activity, filter paper activity and β-glucosidase enzyme activity by 3,5-dinitrosalicylic acid method）. In this way, four strains with high cellulase-producing activity were acquired. The strain 16-7 had the highest cellulase decomposition capacity and most stable enzyme activity, its CMCase activity was 265.76 U/mL, filter paper activity was 86.44 U/mL and β-glucosidase activity was 39.16 U/mL. The strain 16-7 was identified as Penicillium spinulosum by morphology observation and molecular biological identification. |
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| Keywords: | soil high cellulase-producing strain screening enzyme activity |
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