Effect of long-term application of inorganic fertilizer and organic amendments on soil organic matter and microbial biomass in three subtropical paddy soils

Soil organic matter (SOM), microbial carbon (C_mic), and microbial nitrogen (N_mic) status affected by the application of inorganic fertilizer and organic amendments in subtropical paddy soils were investigated. Soil samples were collected from the plow layer of three long-term (17 years) field experiments at Xinhua, Ningxiang, and Taojiang counties in Hunan Province, China. Results showed that, compared to the control, application of inorganic fertilizer alone showed no significant effect on soil organic C (SOC), total N (N_tot), C_mic and N_mic. The application of inorganic fertilizer along with manure or straw significantly increased SOC and N_tot and soil C_mic and N_mic contents for all three sites, while following an application of inorganic fertilizer along with straw only for two sites. C_mic and N_mic were closely correlated with SOC and N_tot, respectively. In conclusion, application of inorganic fertilizer along with manure or straw is an effective way of enhancing SOM and microbial biomass in subtropical paddy soils.