| 乙醇发酵重组大肠杆菌的构建——运动发酵单胞菌pdc基因在大肠杆菌中的高效表达 |
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| 引用本文： | 李学凤,潘亚平,张晶,钱名宇,杨秀山. 乙醇发酵重组大肠杆菌的构建——运动发酵单胞菌pdc基因在大肠杆菌中的高效表达[J]. 太阳能学报, 2006, 27(11): 1120-1123 |
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| 作者姓名： | 李学凤 潘亚平 张晶 钱名宇 杨秀山 |
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| 作者单位： | 首都师范大学生命科学学院,北京,100037 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 为了使大肠杆菌代谢葡萄糖和木糖产乙醇,将运动发酵单胞菌乙醇发酵途径中的关键酶基因丙酮酸脱羧酶基因与质粒载体pGM-T连接转化至大肠杆菌TOP10中。丙酮酸脱羧酶基因在T7启动子的启动下得到了高效表达。构建的重组大肠杆菌不但能利用葡萄糖也能利用木糖产乙醇,在葡萄糖和木糖同时存在时,优先利用葡萄糖。
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| 关 键 词： | 运动发酵单胞菌 丙酮酸脱羧酶基因 重组大肠杆菌 乙醇发酵 |
| 文章编号： | 0254-0096（2006）11-1120-04 |
| 收稿时间： | 2004-12-14 |
| 修稿时间： | 2004-12-14 |
HIGH EXPRESSION OF PYRUVATE DECARBOXYLASE GENE FROM ZYMOMONAS MOBILIS IN ESCHERICHIA COLI |
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| Li Xuefeng,Pan Yaping,Zhang Jing,Qian Mingyu,Yang Xiushan. HIGH EXPRESSION OF PYRUVATE DECARBOXYLASE GENE FROM ZYMOMONAS MOBILIS IN ESCHERICHIA COLI[J]. Acta Energiae Solaris Sinica, 2006, 27(11): 1120-1123 |
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| Authors: | Li Xuefeng Pan Yaping Zhang Jing Qian Mingyu Yang Xiushan |
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| Affiliation: | Gollege of Life Science, Capital Normal University, Beijing 100037, China |
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| Abstract: | To construct a recombinant Escherichia coli strain is able to metabolize glucose and xylose to ethanol,pyruvate decarboxylase gene encoding essential enzyme of the fermentative pathway for ethanol production in Zymomonas mobilis was inserted to plasmid pGM-T and transform into E.coli TOP10.Pyruvate decarboxylase gene was high expressed under the control of T7 promoter.The recombinant E.coli containing this plasmid can ferment both glucose and xylose to product ethanol,and it metabolizes glucose in preference to xylose when glucose and xylose were simultaneously presented. |
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| Keywords: | zymomonas mobilis pyruvate decarboxylase gene recombinant E.coli ethanol fermentation |
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