| 女贞子多糖结构的原子力显微镜研究 |
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| 引用本文： | 徐平平,孙润广,张静,刘娜女,张静,李岱,王小梅. 女贞子多糖结构的原子力显微镜研究[J]. 电子显微学报, 2009, 28(2): 157-161 |
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| 作者姓名： | 徐平平 孙润广 张静 刘娜女 张静 李岱 王小梅 |
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| 作者单位： | 陕西师范大,学物理学与信息技术学院,陕西,西安,710062;陕西师范大学,物理学与信息技术学院,陕西,西安,710062;陕西师范大学,食品工程系,陕西,西安,710062;西安石油大学理学院,陕西,西安,710061 |
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| 基金项目： | 国家自然科学基金，陕西省自然科学基础研究计划 |
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| 摘 要： | 水提女贞子粗多糖经过纯化得到三种多糖分别为LL-Ⅰ1,LL-Ⅰ2,LL-Ⅲ。用气相色谱对女贞子多糖组分进行分析,用原子力显微镜对其结构形态进行观测,结果表明,LL-Ⅰ1,含有鼠李糖/核糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖;LL-Ⅰ2,含有鼠李糖/核糖和葡萄糖;LL-Ⅲ含有鼠李糖/核糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖。原子力显微镜分析表明LL-Ⅰ1,LL-Ⅰ2,LL-Ⅲ分别不同程度的聚集成股,且具有螺旋结构。这种现象可能与聚集体的分子间相互作用和糖链间的氢键缔合有关。
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| 关 键 词： | 女贞子多糖 原子力显微镜 气相色谱 |
Study of structure of polysaccharides from Ligustrum Lucidum Ait by atomic force microscopy |
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| XU Ping-ping,SUN Run-guang,ZHANG Jing,LIU Nanv,ZHANG Jing,LI Dai,WANG Xiao-mei. Study of structure of polysaccharides from Ligustrum Lucidum Ait by atomic force microscopy[J]. Journal of Chinese Electron Microscopy Society, 2009, 28(2): 157-161 |
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| Authors: | XU Ping-ping SUN Run-guang ZHANG Jing LIU Nanv ZHANG Jing LI Dai WANG Xiao-mei |
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| Affiliation: | 1. College of Physics and Information Technology;2. Department of Food Engineering;Shaanxi Normal University;Xi'an Shanxi 710062;3. Xi'an University of Petroleum;Xi'an Shanxi 710061;China |
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| Abstract: | The crude polysaccharide in Ligustrum lucidum Ait was isolated and purified though Sephadex G-150 to yield three water-soluble polysaccharides coded as LL- Ⅰ1, LL-Ⅰ 2, LL-Ⅲ. Monosaccharide composition of LL-Ⅰ 1, LL-Ⅰ 2, LL-Ⅲ were analyzed by Gas Chromatography and the results indicated that LL- Ⅰ 1 mainly consists of Rhamnose/Ribose,Arabinose,Marmose, Glucose and Galctose, LL- Ⅰ 2 mainly consists of Rhamnose/Ribose and Glucose, LL-Ⅲ mainly consists of Rhamnose/Ribose,Arabinose. Mannose Glucose and Galctose. The analysis of AFM results showed that polysaccharide chains of LL- Ⅰ 1, LL- Ⅰ 2, LL- Ⅲ were interweaved in different degree and have the helical structure. The phenomenon may be related to van der Waals interaction and hydrogen bend of polysaccharide chains. |
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| Keywords: | Polysaccharides from Ligustrum lucidum Ait atomic force microscopy gas chromatography |
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