Acidolysis of Tripalmitin with Oleic Acid Catalyzed by a Newly Isolated Thermostable Lipase |
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| Authors: | M Guncheva D Zhiryakova N Radchenkova M Kambourova |
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| Affiliation: | (1) Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;(2) Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria |
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| Abstract: | The catalytic efficiency of a lipase from Bacillus stearothermophilus MC7 (lipase MC7) was evaluated in acidolysis of tripalmitin with oleic acid to yield dioleoylpalmitoylglycerol, a structured
triglyceride used in health food. The immobilized enzyme exhibits good operational thermostability with a half-life of 50 days
at 60 °C in a solvent-free system. The degree of conversion exceeded 50% after 48 h. The side reaction of hydrolysis was suppressed.
However, the monosubstituted product was prevalent in the product mixture. Tested in a broad range of solvents, lipase MC7
showed tolerance towards medium polarity. |
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| Keywords: | Bacillus stearothermophilus MC7 Thermostable lipase Structured lipid Non-solvent acidolysis |
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