Structural changes of sinapic acid during alkali-induced air oxidation and the development of colored substances |
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Authors: | R Cai S D Arntfield J L Charlton |
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Affiliation: | (1) Department of Food Science, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada;(2) Department of Chemistry, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada |
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Abstract: | Structural changes of sinapic acid were induced by air oxidation in aqueous solutions at pH 7–10 and followed by spectral
and high-performance liquid chromatographic (HPLC) analysis. Color properties of the sinapic acid solutions were determined
by taking the transmittance spectra, calculating the Commission Internationale de l’Eclairage (CIE) 1931 tristimulus values,
and converting to Hunter L a b values. Reaction rate constants for sinapic acid were determined by a kinetic study based on
the quantitative results from HPLC analysis. These reactions were first order with respect to sinapic acid and fit the appropriate
equation with a coefficient of R
2 >0.97. Sinapic acid was converted to thomasidioic acid with reaction rate constants (k) of 8.54×10−6, 2.51×10−5, and 4.87×10−5 s−1 in phosphate-boric acid buffers of pH 7, 8.5, and 10, respectively. Similar reactions in ammonium bicarbonate buffers were
more than 10 times faster. With time, thomasidioic acid further converted to 2,6-dimethoxy-p-benzoquinone and 6-hydroxy-5,7-dimethoxy-2-naphthoic acid. Air oxidation of sinapic acid aqueous solutions caused darkening
of the color for the system, with the 2,6-dimethoxy-p-benzoquinone as a major color contributor. |
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Keywords: | Canola phenolics chromatographic analysis color 2 6-dimethoxy-p-benzoquinone 6-hydroxy-5 7-dimethoxy-2-naphthoic acid reaction rate constant sinapic acid thomasidioic acid |
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