陡脉冲致兔肝组织不可逆性电击穿的量效关系

为优化陡脉冲电场在肿瘤治疗的治疗参数,确定最佳的治疗剂量,实验研究了不同电压峰值、脉宽、频率组合的陡脉冲电场致正常兔肝组织细胞不可逆性电击穿范围(即杀伤面积)的量效关系。结果发现:不同参数组合的陡脉冲对杀伤面积有较大的影响,随着脉冲剂量增大,杀伤面积随之增加,但峰值电压约900V时趋于饱和,脉宽>5μs后杀伤面积增大较前明显。

Dosimetry Study on Irreversible Electrical Breakdown of Rabbit Liver Tissue Under Steep Pulsed Electric Field

The application of steep pulse to tumor treatment has been proposed as a promising method.The basic idea of steep pulse therapy is to apply steep pulse to tumors and thus induce irreversible electrical breakdown and apoptosis,namely programmed death,of cancer cells.The application of steep pulse can also induce anti-blood vessel,anti-lymphatic vessel and immune effect and therefore will contribute to destroy life condition of tumors.A lot of research and development work concerning steep pulse therapy has been carried out.It has been proved that steep pulse therapy is an effective and efficient method and thus has a very promising clinical prospect.However,dose-response study of steep pulse therapy is very limited so far.This paper deals with the quantitative study on the parameters of pulsed electric fields and the corresponding damaged area.Studies on lethal effects of steep pulsed electric fields of different parameter combinations(peak value of voltage,pulse width and frequency) on irreversible electrical breakdown of rabbit liver tissue(damaged area) have been conducted.Each pulsed electric field applied to rabbit liver has a different pulse parameter combination.Damaged area is measured and thus the relative dose-response relationship is obtained.Results show that the value of steep pulsed electric fields parameters has a great influence on the damaged area.With the increase of parameter value,a rise in area damaged and also a saturation tendency can be observed.Although the study carried in this paper is quite primary,it can also provide useful information for further clinical studies on steep pulse therapy.