| 采用高效液相色谱法测定牛乳中腐马素含量 |
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| 引用本文： | 马丽艳,贾茹,李景明. 采用高效液相色谱法测定牛乳中腐马素含量[J]. 食品与发酵工业, 2006, 32(1): 72-74 |
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| 作者姓名： | 马丽艳 贾茹 李景明 |
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| 作者单位： | 1. 农业部农产品质量监督检验测试中心,北京,100083;中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 建立了牛乳中腐马素的提取与定量分析方法。样品以甲醇-丙酮体积比1∶1为提取溶剂,利用强阴离子交换柱(SAX)使样品中的腐马素含量达到了浓缩、富集5倍的水平。提取后的样品通过柱前衍生(邻苯二甲醛,OPA)后采用高效液相色谱(HPLC)方法进行检测。采用荧光检测器,以乙腈、水和冰乙酸组成的体系为流动相进行3元梯度洗脱,腐马素FB1和FB2在0.1~2.0μg/mL范围内线性关系良好。FB1和FB2的添加浓度在25~100ng/mL时,FB1的回收率在74.5%~84.1%之间,FB2的回收率在71.7%~82.3%之间,腐马素的最低检出限分别为FB18 ng/mL、FB25 ng/mL。采用文中所建立的腐马素提取和分析方法对市场采集的巴氏消毒牛乳进行了分析,未发现腐马素FB1和FB2。
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| 关 键 词： | 腐马素 高效液相色谱 |
| 收稿时间： | 2005-05-20 |
| 修稿时间： | 2005-12-09 |
Determination Fumonisins in Milk by HPLC |
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| Ma Liyan,Jia Ru,Li Jingming. Determination Fumonisins in Milk by HPLC[J]. Food and Fermentation Industries, 2006, 32(1): 72-74 |
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| Authors: | Ma Liyan Jia Ru Li Jingming |
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| Abstract: | A high-performance liquid chromatograph(HPLC) system to determination fumonisins in milk was described.By this method,the samples were extraction by methanol-acetone,purify on strong anion exchange cartridges.Fumonisins were concentrated five times.Then the samples were derivationed with OPA.and determined by fluorescence detection.The mobile phases was composed with methanolwater-acetic acid.Under such conditions,the recovery of FB1 and FB2 were between 74.5～84.1% and 71.7～82.3%,respectively.The detection limits for FB_1 and FB_2 in milk were 8 ng/g and 5 ng/g,respectively.Pasteurized milk samples collected from supermarkets were detected,and not found FB_1 and FB_2. |
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| Keywords: | fumonisins HPLC |
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