纳米TiO_2光声协同催化损伤牛血清白蛋白的研究

选取牛血清白蛋白为模拟物,研究了纳米二氧化钛光声协同催化作用对BSA的损伤,考察了催化剂用量、初始溶液pH、照射时间等因素对BSA分子损伤的影响。实验结果表明,TiO2光声协同催化作用对BSA的损伤效果明显好于TiO2超声催化或光催化处理,证实了光声联合催化技术具有明显的协同效应。初步探讨了光声协同照射激活纳米TiO2粒子损伤BSA分子的机理,认为是光声协同作用产生高能空穴和羟基自由基所致。

Investigation on Sono-photocatalytic Damage of BSA Molecules Using Nano-TiO2 as a Catalyst

The damage of bovine serum albumin （BSA） molecules in aqueous solution was studied under ultrasonic and/or photocatalytic process using nano-TiO2 as a catalyst. The factors such as quantity of nano-TiO2, solution acidity, irradiation time were tested. The results showed that sono-photocatalysis synergetic technology was superior to each of ultrasound irradiation and photocatalysis alone. The significant synergetic effect of sono-photocatalytic damage of BSA molecules was identified. In addition, the possible mechanism on the damage of BSA molecule in the presence of nano-sized TiO2 powders under sono-photucatalytic process was discussed. It was considered that the damage of BSA molecule was attributed to the formation of photogeneratcd hole and ＂OH radicals resulted from the sono-photocatalysis and high-heat excitation.