| 用随机扩增多态性DNA技术对重离子辐照大丽花花色突变体的初步研究 |
| |
| 引用本文： | 董喜存,李文建,余丽霞,周利斌,马爽,颉红梅.用随机扩增多态性DNA技术对重离子辐照大丽花花色突变体的初步研究[J].辐射研究与辐射工艺学报,2007,25(1):62-64. |
| |
| 作者姓名： | 董喜存 李文建 余丽霞 周利斌 马爽 颉红梅 |
| |
| 作者单位： | 1. 中国科学院近代物理研究所,兰州,730000;中国科学院研究生院,北京,100049
2. 中国科学院近代物理研究所,兰州,730000 |
| |
| 基金项目： | 中国科学院"西部之光"人才培养计划 |
| |
| 摘 要： | 品系为"大雪青"的大丽花幼枝,经兰州重离子加速器提供的80MeV/u 12C6 辐照后,种植在甘肃省定西市临洮新兴花卉公司基地.辐照8Gy的幼枝有一株花色突变体,用随机扩增多态性DNA(Random amplified polymorphic DNA,RAPD)技术对突变体和野生型进行检测分析.琼脂糖凝胶电泳结果表明,所用的10条引物中,有7条引物共扩增出78条带,其中5条引物扩增出了11条多态性片段,从而在DNA水平上证实了两者之间存在着差异,为进一步探讨重离子诱变机理打下基础.
|
| 关 键 词： | 碳离子束 花色突变体 大丽花 随机扩增多态性DNA |
| 收稿时间： | 2006-09-15 |
| 修稿时间： | 2006-11-27 |
Preliminary study on flower color mutant induced by 80MeV/u 12C+6 ions in Dahlia pinnata Cav using RAPD technique |
| |
| DONG Xicun,LI Wenjian,YU Lixia,ZHOU Libin,MA Shuang,XIE Hongmei.Preliminary study on flower color mutant induced by 80MeV/u 12C+6 ions in Dahlia pinnata Cav using RAPD technique[J].Journal of Radiation Research and Radiation Processing,2007,25(1):62-64. |
| |
| Authors: | DONG Xicun LI Wenjian YU Lixia ZHOU Libin MA Shuang XIE Hongmei |
| |
| Affiliation: | 1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000; 2.Graduate School of Chinese Academy of Sciences, Beijing 100049 |
| |
| Abstract: | Young shoots of Dahlia pinnata Cav (Daxueqing) cultivar were irradiated by 80MeV/u 12C6 ions. The as-irradiated shoots were planted in the field of Lintao Flower Corporation in Dingxi City, Gansu Province. Flower color mutant was induced by 8Gy irradiation. Mutant and wild type of Dahlia pinnata Cav were detected for their DNA level differences using RAPD (Random amplified polymorphic DNA) method. The spectra of electropho- resis in agarose gel indicated that among 10 primers used, the 78 bands are produced in 7 primers. Among them 11 polymorphic bands are produced in 5 primers, which substantiated the differences in the DNA level between them. Mutation mechanism by heavy ion beams will be further studied on basis of the results. |
| |
| Keywords: | Carbon ion beam Flower color mutant Dahlia pinnata Cav Random amplified polymorphic DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
