| 芸薹属紫菜蔬乙醇提取物对葡聚糖硫酸钠致小鼠结肠炎的预防作用 |
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| 引用本文： | 吴铭,宋家乐,任源,曾榛,钱波,桂中玉,吕芳芳,庞棋期.芸薹属紫菜蔬乙醇提取物对葡聚糖硫酸钠致小鼠结肠炎的预防作用[J].现代食品科技,2019,35(9):153-162. |
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| 作者姓名： | 吴铭 宋家乐 任源 曾榛 钱波 桂中玉 吕芳芳 庞棋期 |
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| 作者单位： | 桂林医学院公共卫生学院,广西桂林,541100;桂林医学院公共卫生学院,广西桂林541100;CHA医科大学食品生命工学系及生物技术研究所,韩国抱川11160;桂林医学院公共卫生学院,广西桂林541100;釜山大学食品科学与营养学系,韩国釜山609735 |
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| 基金项目： | 国家自然科学基金项目(81560530、81760589)；广西自然科学基金项目(2016GXNSFCA380026)；广西高等学校千名中青年骨干教师培育计划资助项目(桂教人[2018]18号)；桂林医学院引进人才科研启动基金项目(04010150001) |
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| 摘 要： | 本研究探讨紫菜薹(BCEE)、紫甘蓝(RCEE)、紫芜箐(BREE)及羽衣甘蓝(BOEE)乙醇提取物对葡聚糖硫酸钠(DSS,2%)诱发C57BL/6J小鼠溃疡性结肠炎的影响。观察小鼠体重,结肠长度,疾病活性指数及结肠组织变化。试剂盒法检测小鼠结肠髓过氧化物酶(MPO)和超氧化物歧化酶(SOD),过氧化氢酶(CAT),谷胱甘肽还原酶(GSH-Px),谷胱甘肽(GSH)和丙二醛(MDA)水平。酶联法测定肠内炎性因子(TNF-α,IL-1β,IL-6和IL-18)水平。q RT-PCR法检测结肠内炎性因子,NLRP3,ASC,caspase-1,i NOS和COX-2的m RNA水平。较模型小鼠4种紫蔬菜均显著抑制DSS致体重减轻,增加结肠长度至8.67 cm (BCEE组),8.23 cm (BREE组),7.66cm (RCEE组)和7.40 cm (BOEE组);改善肠内炎症程度;提升肠内抗氧化物酶(SOD,CAT和GSH-Px)和GSH水平;降低炎细胞因子(TNF-α、IL-1β、IL-6和IL-18),MPO及MDA水平。此外,紫菜蔬能抑制结肠组织中炎性因子,NLRP3,ASC,caspase-1和i NOS与COX-2的m RNA表达。综上数据提示,4种紫菜蔬缓解DSS诱导小鼠结肠炎能力为BCEEBREERCEEBOEE。
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| 关 键 词： | 紫色菜蔬 葡聚糖硫酸钠 C57BL/6J小鼠 实验性结肠炎 抗炎 |
| 收稿时间： | 2019/4/14 0:00:00 |
Preventive Effects of Purple Brassica Vegetables Ethanolic Extracts in DSS Induced Mice Colitis |
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| WU Ming,SONG Jia-le,REN Yuan,ZENG Zhen,QIAN Bo,GUI Zhong-yu,LYU Fang-fang and PANG Qi-qi.Preventive Effects of Purple Brassica Vegetables Ethanolic Extracts in DSS Induced Mice Colitis[J].Modern Food Science & Technology,2019,35(9):153-162. |
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| Authors: | WU Ming SONG Jia-le REN Yuan ZENG Zhen QIAN Bo GUI Zhong-yu LYU Fang-fang and PANG Qi-qi |
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| Affiliation: | (1.School of Public and Health, Guilin Medical University, Guilin 541001, China),(1.School of Public and Health, Guilin Medical University, Guilin 541001, China)(2.Department of Food Science and Biotechnology, and Institute of Biotechnology, CHA Medical University, Pocheon 11160, Korea),(1.School of Public and Health, Guilin Medical University, Guilin 541001, China),(1.School of Public and Health, Guilin Medical University, Guilin 541001, China),(1.School of Public and Health, Guilin Medical University, Guilin 541001, China),(1.School of Public and Health, Guilin Medical University, Guilin 541001, China),(1.School of Public and Health, Guilin Medical University, Guilin 541001, China) and (1.School of Public and Health, Guilin Medical University, Guilin 541001, China)(3.Department of Food Science and Nutrition, Pusan National University, Pusan 609-735, Korea) |
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| Abstract: | |
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| Keywords: | purple Brassicavegetables dextran sulfate sodium C57BL/6J mice experimentalcolitis anti-inflammation |
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