| 响应面优化微波结合酶法制备米糠多肽过程中黄曲霉毒素B1的降解工艺 |
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| 引用本文： | 林耀盛,林奕云,张惠娜,钟晶晶,刘学铭.响应面优化微波结合酶法制备米糠多肽过程中黄曲霉毒素B1的降解工艺[J].现代食品科技,2019,35(7):173-181. |
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| 作者姓名： | 林耀盛 林奕云 张惠娜 钟晶晶 刘学铭 |
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| 作者单位： | 广东省农业科学院蚕业与农产品加工研究所,农业部功能食品重点实验室,广东省农产品加工重点实验室,广东广州510610;中国广州分析测试中心,广东省分析测试技术公共实验室,广东广州510070 |
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| 基金项目： | 公益性行业（农业）科技专项（201203037-01-01）；国家重点研发计划项目（2017YFD0401105）；广东省省级科技计划项目（2017A030303041；2015A030310466） |
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| 摘 要： | 研究微波结合酶解法组合技术对米糠多肽制备过程中黄曲霉毒素B_1(Aflatoxin B_1,AFB_1)的影响,为染毒米糠的脱毒提供新途径。在单因素试验的基础上,将料液比(X1)、微波时间(X2)、碱性蛋白酶酶解时间(X3)、酶解p H(X4)因素进行组合,应用Design Expert软件和Box-Behnken中心组合试验方法对AFB1降解条件进行了优化,测定米糠多肽AFB1的降解量。初始米糠中AFB1浓度为100.14μg/kg,运用响应面分析确定了其最佳工艺条件:微波功率750 W、微波时间9.56 min;碱性蛋白酶酶解温度50℃、酶解p H 10.74、酶解时间1.57 h、加酶量0.4%;料液比1:9.96,在此条件下,米糠中AFB1的降解率为98.68%,所制备的米糠多肽残留浓度为1.85μg/kg,符合国家标准(≤10μg/kg)。通过响应面优化微波结合酶法制备多肽工艺发挥协同作用,能有效降低米糠多肽AFB1含量满足国家标准限量要求。微波结合酶解处理条件温和,降解效率高、操作方便,可推广应用于受黄曲霉毒素的降解的粮油制备中。
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| 关 键 词： | 米糠多肽 微波处理 酶法 黄曲霉毒素B1 响应面脱除 |
| 收稿时间： | 2019/5/14 0:00:00 |
Study on Degradation of Aflatoxin B1 during the Preparation of Rice Bran Polypeptide by Response Surface Methodology Microwave-assisted Enzyme Treatment |
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| LIN Yao-sheng,LIN Yi-yun,ZHANG Hui-n,ZHONG Jing-jing and LIU Xue-ming.Study on Degradation of Aflatoxin B1 during the Preparation of Rice Bran Polypeptide by Response Surface Methodology Microwave-assisted Enzyme Treatment[J].Modern Food Science & Technology,2019,35(7):173-181. |
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| Authors: | LIN Yao-sheng LIN Yi-yun ZHANG Hui-n ZHONG Jing-jing and LIU Xue-ming |
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| Affiliation: | (1.Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China),(2.Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center, Guangzhou 510070, China),(1.Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China),(1.Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China) and (1.Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China) |
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| Abstract: | |
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| Keywords: | polypeptide of rice bran bran microwave treatment enzymatic pretreatment AFB1 response surface method for detoxification |
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