Fatty acid and sterol synthesis by rat small intestine in vitro |
| |
Authors: | S. G. Miguel |
| |
Affiliation: | (1) Department of Home Economics, Arizona State University, 85281 Tempe, AZ |
| |
Abstract: | Slices of rat jejunum were incubated with [2-14C]pyruvate, [1-14C]acetate, or [3H]H2O to determine lipogenic activity. Under all conditions studied, pyruvate acted as a better precursor than acetate for fatty acid synthesis but not for the synthesis of sterol. Exogenous glucose significantly (P≤0.05) increased the conversion of both pyruvate and acetate to fatty acids. By contrast, fasting resulted in a decrease (p≤0.05) in lipogenic activity. The highest levels of lipogenesis were observed when [3H]H2O + glucose at a concentration of 20 mM was used. From such experiments, the absolute rate of fatty acid synthesis in the tissue preparation was calculated: 734±54 nmoles acetyl units incorporated into fatty acids/g tissue/hr. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|