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| A Novel Fluorescence Probe 9-（4-（1,2-diamine）benzeneN 1-phenyl）acridine for Nitric Oxide Determination | |
| 引用本文： | 丁莉芸,袁芳,梁兵. A Novel Fluorescence Probe 9-（4-（1,2-diamine）benzeneN 1-phenyl）acridine for Nitric Oxide Determination[J]. 武汉理工大学学报(材料科学英文版), 2014, 29(4): 848-853. DOI: 10.1007/s11595-014-1007-3 |
| 作者姓名： | 丁莉芸 袁芳 梁兵 |
| 作者单位： | [1]National Engineering Laboratory for Fiber Optic Sensing Technology, Wuhan University of Technology, Wuhan 430070, China; [2]Department of Health, Qilu Hospital, Shandong University, Qingdao 266071, China; [3]Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China; [4]School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China; [5]Department of Neurology, Qilu Hospital, Shandong University, Jinan 250012, China |
| 基金项目： | Funded by the National Natural Science Foundation of China（Nos.50802069,81100890,51272191）; the Fundamental Research Funds for the Central Unversities（WUT：2013-IV-010）; the Students Innovation and Entrepreneurship Training Program of WHUT（20141049701012） |
| 摘 要： | A novel fluorescent probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA) was synthesized for the detection of nitric oxide(NO) and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship(R2=0.9977) with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT(3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide) assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine. |
| 关 键 词： | 荧光探针 一氧化氮 苯基 二胺 氮测定 最佳实验条件 PC12细胞 L-精氨酸 |
A novel fluorescence probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine for nitric oxide determination |
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| Liyun Ding,Fang Yuan,Lanfen Huang,Jun Huang,Xiaofang Liu,Bing Liang. A novel fluorescence probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine for nitric oxide determination[J]. Journal of Wuhan University of Technology. Materials Science Edition, 2014, 29(4): 848-853. DOI: 10.1007/s11595-014-1007-3 | |
| Authors: | Liyun Ding Fang Yuan Lanfen Huang Jun Huang Xiaofang Liu Bing Liang |
| Affiliation: | 1. National Engineering Laboratory for Fiber Optic Sensing Technology, Wuhan University of Technology, Wuhan, 430070, China 2. Department of Health, Qilu Hospital, Shandong University, Qingdao, 266071, China 3. Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China 4. School of Chemical Engineering, Wuhan University of Technology, Wuhan, 430070, China 5. Department of Neurology, Qilu Hospital, Shandong University, Jinan, 250012, China |
| Abstract: | nitric oxide fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine |
| Keywords: | nitric oxide fluorescent probe 9-（4-（1 2-diamine）benzene-N1-phenyl）acridine |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ | |