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| 简便快速自动化合成报告基因表达PET显像剂18F-FHBG | |
| 引用本文： | 唐刚华,唐小兰,王明芳,李葆元,梁明泉,王全师.简便快速自动化合成报告基因表达PET显像剂18F-FHBG[J].核技术,2009,32(8). |
| 作者姓名： | 唐刚华 唐小兰 王明芳 李葆元 梁明泉 王全师 |
| 作者单位： | 1. 中山大学附属第一医院核医学科PET-CT中心,广州,510080;南方医科大学南方医院核医学科PET中心,广州,510515 2. 华南农业大学理学院,广州,510642 3. 南方医科大学南方医院核医学科PET中心,广州,510515 |
| 基金项目： | 中山大学"百人计划"引进人才科研启动基金，国家高技术研究发展计划(863计划)专题课题 |
| 摘 要： | 用一锅法和TRACERlab FXF-N合成仪自动化合成9-(4-18F-3-羟甲基丁基)鸟嘌呤(188F-FHBG).以N2-(对甲氧苯基二苯基甲基)-9-(4-甲苯磺酰基)-3-对甲氧苯基二苯基甲氧基-甲基丁基]鸟嘌呤为前体,在同一反应瓶中经亲核氟化、水解两步反应及SEP-PAK小柱分离纯化制备18 F-FHBG注射液,总合成时间<40 min,未校正放化产率为7%-12%(n>10),放化纯度>95%.用SEP-PAK分离纯化的一锅法,操作简便,很容易实现18F-FHBG的自动化合成. |
| 关 键 词： | 酶报告基因 自动化合成 |
A simple and rapid automated synthesis of 18F-FHBG as a reporter gene expression PET imaging agent |
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| TANG Ganghua,TANG Xiaolan,WANG Mingfang,LI Baoyuan,LIANG Mingquan,WANG Quanshi.A simple and rapid automated synthesis of 18F-FHBG as a reporter gene expression PET imaging agent[J].Nuclear Techniques,2009,32(8). | |
| Authors: | TANG Ganghua TANG Xiaolan WANG Mingfang LI Baoyuan LIANG Mingquan WANG Quanshi |
| Abstract: | Via one-pot procedure in a TRACERlab FXF-N synthesizer,the fully automated synthesis of 9-(4-18F]fluoro-3- hydroxymethylbutyl)guanine (18F-FHBG) as a reporter gene expression PET imaging agent is described in this paper.18F-FHBG was produced via two-step reactions including nucleophilic fluorination of the precursor molecule N2-monomethoxy trityl-9-(4-tosyl)-3-monomethoxytrityl-methylbutyl] guanine with 18F-fluoride and subsequent hydrolysis of the intermediate with protecting group with 1 mol/L HC1 at the same reaction vessel,and purification with SEP-PAK C18 cartridge.The decay-uncorrected radiochemical yield of 18F-FHBG was 7%-12% (n>10),with radiochemical purity of over 95%,and the total synthesis time was less than 40 min.One-pot synthesis procedure combined with SEP-PAK purification is easy to operate and can be used to fully automatedsynthesis of 18F-FHBG at an automated system such as a TRACERlab FXF-N synthesizer. |
| Keywords: | 18F-FHBG Enzyme reporter gene 18F-FHBG Automated synthesis |
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