Optimizing cholesterol removal from cream using β-cyclodextrin cross-linked with adipic acid

The present study was carried out to optimize the different conditions for cross-linked β-cyclodextrin (β-CD) using adipic acid on cholesterol removal in cream. Different factors were β-CD concentration, mixing temperature, mixing time and mixing speed. Cross-linked β-CD was prepared with adipic acid. When the cream was treated with 10% cross-linked β-CD, cholesterol removal was the highest at 90.7%, which was not significantly different from treatments with 15% and 20% cross-linked β-CD. Cholesterol removal was significantly influenced by mixing temperature, mixing time and mixing speed. After cholesterol removal from cream, the used cross-linked β-CD was washed for cholesterol dissociation and reused. For recycling study, the cholesterol removal in first trial was 91.5%, which was mostly same as that using new cross-linked β-CD. However, after 10 repeated trials using the same sample, only 83.4% of the cholesterol was removed from cream. Therefore, the present study indicated that the optimum conditions for cholesterol removal using cross-linked β-CD were 10%β-CD addition, 30 min mixing with 1400 r.p.m. speed at 40°C with over 90% cholesterol removal. In addition, recycled β-CD cross-linked with adipic acid showed a similarly high recycling efficiency to unused β-CD of about 90% up to the seventh trial.