| 大孔吸附树脂分离纯化番石榴叶总皂苷的工艺研究 |
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| 引用本文： | 马连荣,黄丽秀,欧丽兰,田芳年,莫肖云,黄锁义. 大孔吸附树脂分离纯化番石榴叶总皂苷的工艺研究[J]. 化工技术与开发, 2009, 38(8): 4-6 |
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| 作者姓名： | 马连荣 黄丽秀 欧丽兰 田芳年 莫肖云 黄锁义 |
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| 作者单位： | 右江民族医学院2005级检验本科班,广西,百色,53300;右江民族医学院药学系,广西,百色,53300 |
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| 基金项目： | 右江民族医学院2007-2009年度普高学生科研项目立项资助课题 |
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| 摘 要： | 研究大孔吸附树脂分离纯化番石榴叶总皂苷的工艺条件.番石榴叶用70%乙醇回流提取后,上D101型大孔树脂,水洗后分别用30%,50%,70%乙醇洗脱,以番石榴叶总皂苷的洗脱率为指标,考察大孔树脂分离纯化番石榴叶总皂苷的吸附性能和洗脱参数.番石榴叶总皂苷主要富集于30%、50%乙醇洗脱液部分,大孔吸附树脂的吸附容量为17.53 mg·g-1,洗脱率达70.42%,而50%乙醇洗脱时总皂苷纯度可达55.68%,优选洗脱条件为用水洗去水溶性杂质,50%乙醇洗脱总皂苷.
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| 关 键 词： | 大孔吸附树脂 番石榴叶 总皂苷 提取工艺 |
Separation and Purification of Total Saponins from Guava Leaf with Macroporous Absorptive Resin |
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| MA Lian-rong,HUANG Li-xiu,OU Li-lan,TIAN Fang-nian,MO Xiao-yun,HUANG Suo-yi. Separation and Purification of Total Saponins from Guava Leaf with Macroporous Absorptive Resin[J]. Technology & Development of Chemical Industry, 2009, 38(8): 4-6 |
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| Authors: | MA Lian-rong HUANG Li-xiu OU Li-lan TIAN Fang-nian MO Xiao-yun HUANG Suo-yi |
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| Affiliation: | 1.Medical Science of 2005 Grades Examines the Undergraduate Course;Youjiang Medical College for Nationalities;Baise 533000;China;2.Department of Pharmacy;China |
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| Abstract: | A method for the separation and purification of total saponins from guava leaf by column chromatography of macroporous absorptive resin was established.Radix clematidis was extracted with 70% ethanol and then the extraction was purified with D101 macroporous absorptive resin.After eluting with distilled water and 30%,50% and 70% ethanol respectively,the absorption capability and elution parameters of macroporous absorptive resin were measured by the indexes of elution rate and refinement rate of total sapon... |
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| Keywords: | macroporous absorptive resin guava leaf total saponins extraction technique |
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