| Affiliation: | 1. Center for AIE Research, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060 China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060 China;2. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072 China;3. Center for AIE Research, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060 China;4. Hong Kong Branch of Chinese National Engineering Research, Center for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, 999077 China |
| Abstract: | Graphene oxide (GO)-based fluorescent DNA aptasensors are promising nanomaterials in bioassays owing to the fluorescent ultrasensitivity and target identification ability. However, their in vivo application remains an appealing yet significantly challenging task. In this contribution, for the first time, a nanomaterial for in vivo diagnosis and therapy of liver tumors is demonstrated. A DNA nanomaterial consisting of DNA tetrahedron and aptamers, aggregation-induced emission luminogens, and antitumor drug doxorubicin, is fabricated and attached on the GO surface. This developed hybrid with good biocompatibility exhibits high selectivity to target liver cancer cells, and performs well in in vitro and in vivo liver tumor fluorescence imaging diagnosis and chemotherapy. Additionally, a GO-based fluorescent DNA nanodevice is also constructed by using microfluidic chips for liver tumor cell screening. |
