| 抗急性非淋巴细胞白血病M_5型单克隆抗体研究 |
| |
| 引用本文： | 张权一,潘荆华,李秀芝,张颖超,吴铁南,王诚,王晓薇,李长平,易永林.抗急性非淋巴细胞白血病M_5型单克隆抗体研究[J].粉末涂料与涂装,1991(4). |
| |
| 作者姓名： | 张权一 潘荆华 李秀芝 张颖超 吴铁南 王诚 王晓薇 李长平 易永林 |
| |
| 作者单位： | 卫生部长春生物制品研究所 长春130062(张权一，潘荆华，李秀芝，张颖超，吴铁南，王诚)，白求恩医科大学第一临床学院 长春130021(王晓薇，李长平，易永林) |
| |
| 摘 要： | 用急性非淋巴细胞白血病(急非淋)M_5型白血病相关抗原免疫 BALB/C 小鼠,取免疫鼠脾细胞与 NS-1骨髓瘤细胞融合,建立了2株能稳定分泌抗急非淋 M_5型白血病抗体的杂交瘤株。所分泌的单克隆抗体与急非淋 M_5型白血病有反应,与其他型白血病没有交叉反应,具有很好的特异性。CFU-GM 培养结果对骨髓造血干细胞没有影响。
|
| 关 键 词： | 急性非淋巴细胞白血病 杂交瘤 单克隆抗体 |
Research on M_5-type Monoclonal Antibody Against Acute non-Lymphocytic Leukemia |
| |
| Zhang Quanyi Changchun Institute of Biological Products,Changchun.Research on M_5-type Monoclonal Antibody Against Acute non-Lymphocytic Leukemia[J].Chinese Journal of Biologicals,1991(4). |
| |
| Authors: | Zhang Quanyi Changchun Institute of Biological Products Changchun |
| |
| Abstract: | BALB/C mice were immunized with the M_5-type associated antigen of acute non-lymphocytic leukemia,The spleen cells were fused with NS-1/1.Aq.4.1 m yeloma cells, and 2 cell strains which could secret anti-acute non-lymphocytic leukemia M_5-type monocl- onal antibodies were established.All the monoclonal antibodies secreted by these cell stra- ins gave positive reaction with the leukemia cells from acute non-lymphocytic leukemia M_2- type patient,but could not give any reaction with the cells from other type of leukemia patients.Themonoclonal antibodies produced by the cell strains showed good specificity.The result of CFu-GM culture did not affect myeloid stem cells. |
| |
| Keywords: | Acute non-lymphocytic leukemia Hybridoma Monoclonal antibody |
| 本文献已被 CNKI 等数据库收录！ |
