Chemiluminescent determination of cholesterol hydroperoxides in human erythrocyte membrane |
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Authors: | Junko Adachi Migiwa Asano Takeaki Naito Yasuhiro Ueno Yoshitsugu Tatsuno |
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Affiliation: | (1) Kobe Pharmaceutical University, Kobe, Japan;(2) Department of Legal Medicine, Kobe University School of Medicine, 7-5-1 Kusumoki-cho, Chuoku Kube 650-0017, Japan |
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Abstract: | A method for separating, detecting, and quantifying cholesterol hydroperoxide (Ch-OOH) based on extraction, purification by solid-phase extraction cartridge, high-performance liquid chromatography with chemiluminescent detection (HPIC-CI), and liquid chromatography mass spectrometry has been developed for human erythrocyte membrane. We prepared standard compounds of the cholesterol 5α-, 7α-, and 7β-hydroperoxides (Ch 5α-OOH, Ch 7α-OOH, and Ch 7β-OOH). An octyl silica column with methanol/water/acetonitrile 89∶9∶2 (by vol) as eluent was used to determine Ch-OOH. HPLC-CL that incorporated cytochrome c and luminol as the post-column luminescent reagent was used. We also investigated the optimal assay conditions and how to prevent formation of artifact Ch-OOH. Analysis of erythrocyte membranes from seven healthy volunteers identified Ch 7α-OOH and Ch 7β-OOH, but not Ch 5α-OOH, as commonly occurring components. The respective mean concentrations of Ch 7α-OOH and Ch 7β-OOH were 2,5±1.6 and 5A±3.5 pmol/mL blood. |
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