| Polyaniline-graphite composite film glucose oxidase electrode |
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| 作者姓名： | 周海晖 陈宏 陈金华 旷亚非 |
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| 作者单位： | College of Chemistry and Chemical Engineering Hunan University，College of Chemistry and Chemical Engineering Hunan University，College of Chemistry and Chemical Engineering Hunan University，College of Chemistry and Chemical Engineering Hunan University，Changsha 410082 ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics Hunan UniversityChangsha 410082 China，Changsha 410082 China，Changsha 410082 ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics Hunan UniversityChangsha 410082 China，Changsha 410082 ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics Hunan UniversityChangsha 410082 China |
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| 基金项目： | 国家自然科学基金;湖南省科研项目;湖南大学博士基金 |
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| 摘 要： | 1 INTRODUCTION Conducting polymers, such as polyaniline (PANI), polypyrrole (PPY) and polythiophene, have attracted much attention as an enzyme-immobilizing matrix for biosensors 1?3]. Conducting polymers are known to be compatible with biological molecules in neutral aqueous solutions. Due to the reversibility doping-undoping processes and the rapid electron transfer, the conducting polymers are promising in the immobilization of biomolecules and fabrication of efficient biosensors4]…
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| 收稿时间： | 15 March 2006 |
| 修稿时间： | 20 May 2006 |
Polyaniline-graphite composite film glucose oxidase electrode |
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| Zhou Hai-hui , Chen Hong , Chen Jin-hua and Kuang Ya-fei.Polyaniline-graphite composite film glucose oxidase electrode[J].Journal of Central South University of Technology,2006,13(6):653-657. |
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| Authors: | Zhou Hai-hui Chen Hong Chen Jin-hua and Kuang Ya-fei |
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| Affiliation: | (1) College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China;(2) State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, China |
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| Abstract: | A novel polyaniline-graphite composite film glucose oxidase (PGCF GOD) electrode was developed. The PGCF was synthesized by
cyclic voltammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the
composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning
electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure
and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of
pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The
sensor has a maximum steady-state current density of 357.17 μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under
the condition of pH 5.5, 0.8 V and 65 °C.
Foundation item: Projects(50473022, 20673036) supported by the National Natural Science Foundation of China; project(2005) supported by the
State Key Laboratory of Chemo/Biosensing and Chemometrics of China; project(2006FJ4100) supported by the Science Technology
Project of Human Province; project(2006) supported by the Postdoctor Foundation of Hunan University |
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| Keywords: | polyaniline graphite composite film glucose oxidase biosensor |
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