Investigations on the growth ofRhodococcus rubra in relation to the formation of stable biological foams |
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Authors: | A R Khan Kyung-Sok Min Yong-Jun Jung C F Forster |
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Affiliation: | (1) PCSIR Laboratories, Jamrud Road, 25120 Peshawar, Pakistan;(2) Department of Environmental Engineering, Kyungpook National University, 702-701 Taegu, Korea;(3) School of Civil Engineering, University of Birmingham, B 15 2TT Edgbaston, Birmingham |
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Abstract: | The growth characteristics of a foam-forming species,Rhodococcus rubra were studied on different substrates. The basic medium contained Czapek (3.34%), yeast extract (0.2%), potassium dihydrogen
phosphate (0.12%), dipotassium hydrogen phosphate (0.25%) and ammonium chloride (0.1%). This was supplemented with varying
concentrations of glucose (0–2%). The same basic medium was also used to examine the growth ofR. rubra in combination with varying concentration of n-hexadecane (0.0–0.5%) as a source of energy while varying the concentration
of ammonium chloride in the range 1–3 gl
−1. Studies based on determining the biomass concentration, the surface activity related to the cell suspensions and measuring
the variations in broth pH revealed that glucose encouraged the growth ofR. rubra, compared to the control. However, increasing the glucose concentration from 0.1 to 2.0% had no further effect on growth.
The surface activity of the cell suspensions increased with increasing glucose concentration. Results similar to glucose were
exhibited by the addition of n-hexadecane, suggesting same degree of growth among different concentrations with higher surface
activity increasing with increase in substrate concentration. Results have also shown that the pH of all the culture broths
decreased as the ammonium chloride concentration increased, suggesting that there was a production of hydrogen ions during
the course of its metabolism. |
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Keywords: | Wastewater Treatment Stable Foam Rhodococcus rubra Surface Activity |
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