Acceptability of diets containing olive oil fried sardines (Sardina pilchardus) in the prevention of dietary hypercholesterolaemia in rats

Garlic (Allium sativum L) bulb explants produced undifferentiated white globular callus when grown on MS medium having 2,4-dichlorophenoxy-acetic acid (2,4-D) and Kinetin (Kn). Such callus when grown on medium having indole-3-yl-acetic acid (IAA) and/or Kn developed into the differentiating callus which had comparatively more allicin than that of undifferentiated callus. The effect of sucrose, NH/NO ratios and hormone concentrations on growth and allicin development in the callus was investigated. There was a significant increase in allicin development in callus when grown for up to 4 weeks on medium (a) having sucrose at 3% and 5% levels; (b) having NH and NO ratios at 2:1 and 1;1; and (c) with Kn alone at a concentration of 0.5 mg litre^?1, which produced a tenfold increase in allicin content.