Early results using high-resolution,low-voltage,low-temperature SEM |
| |
Authors: | James B Pawley Paul Walther Shian-Jiun Shih Marek Malecki |
| |
Abstract: | Recent advances in the design of the scanning electron microscope (SEM) column, such as the coupling of a field-emission gun to a low-aberration immersion lens and the availability of a high-stability cryo-transfer stage, make low-temperature, low-voltage SEM (LTLVSEM) possible at very high resolution. We have used this combination to obtain results with uncoated biological specimens. The trichocyst from a Paramecium was used as a test specimen to observe the shrinkage of this structure as the temperature is raised from 170 K to room temperature following freeze-drying. High-magnification stereo images were obtained of trichocysts that had been prepared by freezing, freeze-substitution and critical-point drying and which were subsequently viewed by LTLVSEM to reduce beam damage and contamination. |
| |
Keywords: | Low-voltage SEM freezing cryo-SEM high-resolution SEM |
|
|