| 毛细管电泳法检测牛奶中硫氰酸钠质量浓度 |
| |
| 引用本文： | 吴剑平,顾欣,李丹妮,严凤,张鑫. 毛细管电泳法检测牛奶中硫氰酸钠质量浓度[J]. 中国乳品工业, 2012, 40(3): 53-56 |
| |
| 作者姓名： | 吴剑平 顾欣 李丹妮 严凤 张鑫 |
| |
| 作者单位： | 上海市兽药饲料检测所,上海,201103 |
| |
| 摘 要： | 运用毛细管区带电泳法直接检测牛奶中硫氰酸钠质量浓度。前处理使用乙睛沉淀蛋白,中性氧化铝SPE柱除去部分水溶性维生素、氨基酸,离心脱脂。在NaH2PO4-Na2B4O7缓冲液中加入溴化十四烷基三甲基溴化铵(TTAB)作为电渗流反相作用剂,分离电压20 kV,分离温度30℃,电极反向,以二极管阵列紫外检测器218 nm波长下分析样品,质量浓度范围在5~500 mg/L内线性较好,r>0.9995。该方法可适用于生鲜乳和巴氏灭菌成品乳,检测限可达到0.2 mg/kg,定量范围0.5～50 mg/kg,精确度<10%,回收率>90%。
|
| 关 键 词： | 硫氰酸钠 毛细管区带电泳法 电渗流反向作用剂 |
Determination of sodium thiocyanate in milk by CE |
| |
| WU Jian-ping , GU Xin , LI lDan-ni , YAN Feng , ZHANG Xing. Determination of sodium thiocyanate in milk by CE[J]. China Dairy Industry, 2012, 40(3): 53-56 |
| |
| Authors: | WU Jian-ping GU Xin LI lDan-ni YAN Feng ZHANG Xing |
| |
| Affiliation: | g(Shanghai Institute for Veterinary Drug & Feedstuffs control,Shanghai 201103,China) |
| |
| Abstract: | A method to determinate sodium thiocyanate in milk by CE was established.The separation was performed on NaH2PO4-Na2B4O7 with TTAB used as the electro-osmotic flow(EOF) modifier to change the direction of EOF.The detection wavelength was 218 nm.The separate votege was 20 kV and the separate temperature was 30 ℃.Good linearity was showed within the range of 5～500 mg/L respectively,r > 0.9995.The average recoveries was larger than 90.0% respectively,RSD<10%.The method was simple,rapid and reliable for the simultaneous determination of sodium thiocyanate in milk with the LOD of 0.2 mg/kg and the determination range of 0.5～50 mg/kg. |
| |
| Keywords: | sodium thiocyanate CE EOF modifier |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
