响应面法优化大黄游离总蒽醌的提取工艺

优化大黄游离总蒽醌的提取工艺,为其在农业、医药、食品等方面的开发提供理论参数。在单因素试验基础上,选取提取时间、溶剂配比和液料比3个因素进行Box-Benhnken响应面法试验设计,对其提取工艺参数进行优化。在提取温度60℃,提取次数2次,提取时间2.11 h,溶剂配比0.43,液料比23.23时,大黄游离总蒽醌的理论提取率最高,可达1.021%。根据实际试验情况,将其参数修正为提取时间2.1 h,溶剂配比0.4,液料比23.2,提取温度60℃,提取次数2次,经验证此条件下大黄游离总蒽醌含量为1.017%,与理论值较为接近。采用Box-Behnken响应面法建立的游离总蒽醌提取工艺模型提取率高,并能很好地预测试验结果。

Optimization of Extraction Technique of Total Free Anthraquinone from Rheum via Response Surface Methodology

The extraction technique of total free anthraquinone from Rheum was optimized to provide theoretical parameters for its exploitation in agriculture,medicament,foodstuff and so on.On the basis of single-factor experiments,the effects of different time,solvent match and ratio of water to material on the extraction yield of total free anthraquinone from Rheum were investigated with a three-factor Box-Behnken design.The extraction parameters were optimized with Response Surface Methodology.The results indicated that the conditions of 60 ℃,two of the repetition times,refluxing for 2.11 h,23.23∶1 of the ratio of water to material and 0.43 of solvent match were optimum,and the yield of total free anthraquinone could be up to 1.021%.According to conditions of actual experiment,the optimum extraction parameters were modified at 60 ℃,two of the repetition times,refluxing for 2.1 h,23.2∶1 of the ratio of material to water and 0.4 of solvent match.The validated experiment showed the yield of total free anthraquinone was 1.017 % under those conditions which was in consistency with the theoretic value.Extraction model of total free anthraquinone from Rheum established by Box-Behnken method can precisely predict the experiment results.In the best conditions deduced from the mode,the extraction rate of total free anthraquinone is high.