| 脱脂米糠蛋白和多糖的脉冲超声辅助提取技术研究 |
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| 引用本文： | 李前进,马海乐,李树君,李云亮,曲文娟.脱脂米糠蛋白和多糖的脉冲超声辅助提取技术研究[J].中国粮油学报,2011,26(8):5-10. |
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| 作者姓名： | 李前进 马海乐 李树君 李云亮 曲文娟 |
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| 作者单位： | 1. 江苏大学食品与生物工程学院,镇江,212013
2. 中国农业机械化科学研究院,北京,100083 |
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| 基金项目： | 科技部国际合作项目(2009DFA32000) |
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| 摘 要： | 采用脉冲超声技术对脱脂米糠中蛋白和多糖进行同时提取,研究了液料比、提取时间、提取温度、超声发出时间和间歇时间因素对蛋白得率和纯度以及多糖得率和纯度的影响。在单因素试验的基础上,采用Box-Benhnken中心组合试验设计对脉冲超声辅助提取条件进行优化。试验结果表明:脱脂米糠蛋白和多糖的脉冲超声辅助提取最佳条件为:液料比9.5 mL/g,提取时间42.6 min,提取温度51.5℃,超声发出时间4 s,超声间歇时间2 s。在此最佳条件下,脱脂米糠蛋白和多糖的得率分别为3.0%和4.6%,纯度分别为45.6%和76.8%。
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| 关 键 词： | 脱脂米糠 蛋白 多糖 超声提取 响应面 |
Study on Pulsed Ultrasound-assisted Extraction of Protein and Polysaccharide from Defatted Rice Bran |
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| Li Qianjin,Ma Haile,Li Shujun,Li Yunliang,Qu Wenjuan.Study on Pulsed Ultrasound-assisted Extraction of Protein and Polysaccharide from Defatted Rice Bran[J].Journal of the Chinese Cereals and Oils Association,2011,26(8):5-10. |
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| Authors: | Li Qianjin Ma Haile Li Shujun Li Yunliang Qu Wenjuan |
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| Affiliation: | Li Qianjin1 Ma Haile1 Li Shujun2 Li Yunliang1 Qu Wenjuan1 (School of Food and Biological Engineering,Jiangsu University1,Zhenjiang 212013)(Chinese Academy of Agricultural Mechanization Sciences2,Beijing 100083) |
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| Abstract: | The pulsed ultrasonic technology was adopted to extract protein and polysaccharide from defatted rice bran.This research evaluated the effects on the yields and purities of protein and polysaccharide of extraction factors,including liquid to solid ratio,extraction time,extraction temperature,pulsed duration and interval times.Based on the single factor experiment,the response surface analysis was used to optimize the simultaneous extraction condition of protein and polysaccharide by the pulsed ultrasound-as... |
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| Keywords: | defatted rice bran protein polysaccharide ultrasonic extraction response surface analysis |
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