响应面优化超声波辅助提取油茶花中总酚工艺研究

本研究以海南油茶花为原料,运用超声波辅助,响应面法优化提取海南油茶花中总酚,从而获得海南油茶花总酚提取的最佳条件。通过选取以料液比、提取剂乙醇体积分数、超声温度、超声时间为参数自变量,采用Box-Behnken中心组合法,以海南油茶花总酚为响应值,探究4种参数自变量对海南油茶花中总酚提取得率的影响。结果表明,超声波辅助获取油茶花总酚的最佳工艺条件为:料液比1∶600(g/mL),乙醇溶剂体积分数30%,超声温度60℃,超声时间20min,在此最优条件下验证试验油茶花的总酚平均提取得率为(13.03±1.34)%。优化海南油茶花总酚超声波辅助提取的工艺条件,对应用于工业生产及油茶花的综合开发利用具有重要意义。

Optimization of ultrasonic-assisted extraction process of Camellia vietnamensis flower polyphenols using response surface methodology

Using Camellia vietnamensis flower from Hainan as raw material,ultrasonic-assist response surface method was used to optimize the extraction of total phenol in Camellia v.flower so as to obtain the optimal conditions for the extraction of total phenol.Four parameters were selected based on the material-liquid ratio,the extractant ethanol volume fraction,the ultrasonic temperature,and the ultrasonic time.The Box-Behnken central combinatorial test design method was used to explore the effects of four parameters on the yield of total phenol in Camellia v..The results showed that when using the density of 30%ethanol as the extractant,the ratio of material to liquid is 1∶600(g/mL),the ultrasound temperature and time respectively is 60 degrees and 20min,the yield of Camellia v.flower polyphenols can be calculated as(13.03±1.34)%.Thus,it is of great remarkable to optimize the technological conditions of ultrasonic-assisted extraction of total phenols from Camellia v.flower for industrial production and comprehensive development utilization.