山莴苣苦素改善FFA诱导的HepG2细胞脂肪变性及氧化应激的研究

目的:研究山莴苣苦素对游离脂肪酸(FFA)诱导的HepG2细胞脂肪变性的改善作用,并初步探讨其可能作用机制.方法:观察山莴苣苦素对HepG2细胞活力的影响.采用FFA诱导培养HepG2细胞,构建脂肪变性模型(NAFLD体外模型),并用山莴苣苦素干预72h后,检测各组细胞内脂滴数量、甘油三酯(TG)和总胆固醇(TC)含量...

Study on improving FFA induced steatosis and oxidative stress of HepG2 cells by lactucopicrin

Objective:To study the ameliorative effect of Lactucopicrin on FFA-induced steatosis in HepG2 cells and explore its possible mechanism.Methods:The effect of Lactucopicrin on the viability of HepG2 cells was observed.HepG2 cells were induced and cultured with FFA to construct the steatosis model(NAFLD model in vitro).After 72h intervention with Lactucopicrin,the number of lipid droplets,the content of triglyceride(TG)and total cholesterol(TC)as well as the activity of superoxide dismutase(SOD),the content of reduced glutathione(GSH),malondialdehyde(MDA)and the expression of lipid droplet coating protein(Perilipin3)were measured.Results:Compared with the model group,Lactucopicrin intervention significantly reduced(P<0.05)the number of lipid droplets,TG and TC contents,and increased considerably(P<0.05)SOD and GSH levels,and significantly decreased(P<0.05)MDA content and Perilipin3 expression levels in a dose-dependent manner.Conclusions:Lactucopicrin significantly reduced the number of lipid droplets,TG,TC,and MDA contents in FFA-HepG2 cells,increased SOD and GSH levels considerably,and effectively improved FFA-induced steatosis and oxidative stress in HepG2 cells.Therefore,its mechanism of action may be related to altering the process of lipid metabolism and alleviating the level of oxidative stress in the cells.