超声波辅助酶解对玉米醇溶蛋白酶解产物抗氧化及ACE抑制活性的影响

作为玉米加工的副产物,玉米醇溶蛋白可以在酶的作用下形成具备生物活性的多肽物质,应用于药物的生产加工过程.而常规的酶解工艺存在酶的利用率低下、产物转化率低、耗费时间长等缺陷,本文研究了超声波辅助酶解对玉米醇溶蛋白酶解产物抗氧化及ACE抑制活性的影响.研究结果表明,超声功率为200W,超声时间为40min,料液比为1 ∶1...

Effect of ultrasound-assisted enzymatic hydrolysis on the antioxidant and ACE inhibitory activities of zein hydrolysates

As a by-product of corn processing,zein can form biological active peptides under the action of enzymes,which can be used in the process of medicines.However,the conventional enzymatic hydrolysis process has disadvantages such as low utilization rate of enzymes,low product conversion rate,and long time-consuming.This article studied the effect of enzymatic hydrolysis on the antioxidant and ACE inhibitory activities of zein hydrolysates.The results showed that the best degree of hydrolysis of zein could be obtained under the conditions of ultrasonic power 200W,the ultrasonic time 40min,and the material-to-liquid ratio 1∶100.Ultrasound-assisted enzymolysis greatly reduced the particle size of zein hydrolysates,improve the uniformity of zein hydrolysates.After ultrasound-assisted enzymolysis,the soluble protein content was 0.89mg/mL,reducing power was 0.007,sulfhydryl content was 1.354μmol/g,DPPH scavenging ability was 41.39%and ACE inhibitory activity was 63.97%.Compared with the control,these values were increased by 345%,75%,41.63%,247.81%and 53.63%respectively.This study provided a scientific basis for the research and utilization of the zein.