Detection of rennet whey solids in UHT milk by capillary electrophoresis |
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| Affiliation: | 1. Department of Diagnostic Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030;2. Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, Texas;3. Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas;4. Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas;5. Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas;1. School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia;2. Healthy Mothers, Babies and Children theme, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA 5000, Australia;3. Neonatal Services, Women''s and Children''s Health Network, Adelaide, SA 5000, Australia;4. Women''s and Children''s Health Research Institute, North Adelaide, SA 5000, Australia;5. Discipline of Paediatrics, School of Medicine, University of Adelaide, Adelaide SA 5000, Australia;6. Nutrition and Dietetics, Flinders University, Adelaide, SA 5001, Australia |
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| Abstract: | The fraudulent presence of rennet whey solids in UHT milk was studied by capillary electrophoresis (CE). Commercial UHT samples of different origins, genuine milk samples and milk samples adulterated with rennet whey were analysed. Linear discriminant functions using ratios of peak areas of caseinmacropeptide (CMP) and two other CMP-like degradation products were defined. The interference of proteolysis in the detection was estimated in samples adulterated on purpose with rennet whey, UHT treated in a pilot plant, and stored at 10, 20 and 30°C for up to 150 days. The application of the classification functions obtained allowed the detection of rennet whey solids added to milk. Only interferences due to very severe proteolysis, occurring after very long storage periods and/or at storage temperatures above room temperature, were observed. |
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