高效液相色谱分析蓝莓发酵液中鞣花酸和没食子酸

建立了高效液相色谱法测定蓝莓发酵液中鞣花酸含量和没食子酸含量的两种方法。鞣花酸的测定条件：Waters symmetry C18色谱柱,柱温25℃,流动相为46∶2∶52的甲醇—乙酸乙酯—KH2PO4/H3PO4溶液,流速0.8m L/min,检测波长254nm,检测蓝莓发酵液中鞣花酸平均回收率为92.68%,RSD为0.97%。没食子酸的测定条件：Waters symmetry C18色谱柱（250×4.6mm,5μm）,柱温35℃,流动相为甲醇：水（0.1%磷酸）=10∶90,流速为1.0m L/min,检测波长273nm,该方法检测蓝莓发酵液中没食子酸平均回收率为99.0%,RSD为0.73%。两种方法均操作简单快速,重现性好,准确度高,可分别用于香菇等食用菌发酵蓝莓制品中鞣花酸和没食子酸含量的测定。

HPLC Determination of Ellagic Acid and Gallic Acid in Fermented Blueberry

Two methods of High-performance Liquid Chromatography were established to determinate the ellagic acid content and the gallic acid content in fermented blueberry.The measurement conditions of ellagic acid were Waters symmetry C18column（250 × 4.6mm,5μm）,column temperature25℃,the mobile phase 46∶2∶52 methanol-ethyl acetate-KH2PO4/H3PO4 solution,flow rate 0.8 m L/min,and detection wavelength 254 nm,the average detection recovery rate of ellagic acid in blueberries fermentation was 92.68%,RSD was0.97%.The gallic acid measurement conditions of were Waters symmetry C18column（250 × 4.6mm,5μm）,the column temperature35℃,the mobile phase was methanol：water（0.1% phosphoric acid） 10∶90,flow rate 1.0 m L/min,detection wavelength 273 nm,the average detection recovery rate of gallic acid in blueberries fermentation was 99.0%,RSD was 0.73%.Both the methods were simple,fast and reproclucible,and could determine the content of ellagic acid and gllic acid in fermented blueberry accurately.