Determination of fungal microbiota and mycotoxins in brewers grain used in dairy cattle feeding in the State of Bahia,Brazil |
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| Affiliation: | 1. National Food Administration, P.O. Box 622, SE-751 26 Uppsala, Sweden;2. Center for Analytical Chemistry, Department IFA-Tulln, University of Natural Resources and Life Sciences, Vienna, Austria;3. Lantmännen Business development, Grain, SE-531 87 Lidköping, Sweden;1. Dipartimento di Medicina clinica, Sanità Pubblica, Scienze della Vita e dell''Ambiente, Università degli Studi dell''Aquila, Piazzale Salvatore Tommasi 1, Blocco 11, Coppito, 67010 L''Aquila, Italy;2. Laboratorio Innovazione Agroindustriale, Unità Tecnica Sviluppo Sostenibile ed Innovazione del Sistema Agro-industriale, ENEA C.R. Casaccia, Via Anguillarese 301, 00123 Roma, Italy;3. Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per lo Studio delle Relazioni tra Pianta e Suolo, Roma, Italy;4. Dipartimento di Biologia Ambientale, Università degli Studi “Sapienza”, Largo Cristina di Svezia, 24, 00165 Roma, Italy;2. Department of Animal Sciences, Federal University of Lavras, Lavras, MG, Brazil 32700-000;3. Range Cattle Research & Education Center, University of Florida, Ona 33865 |
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| Abstract: | The aim of this study was to determine the mycoflora and evaluate the presence of aflatoxins and ochratoxins in brewers grain used to feed dairy cattle in the State of Bahia. Twenty samples of brewers grain were collected each trimester, during a whole year, in five properties located in cities of the “recôncavo baiano” (Bahia, Brazil) for a total of 80 samples. Samples were analyzed for aflatoxins and ochratoxins by fluorimetry with immunoaffinity columns. Aspergillus was the most frequently isolated genus (42.5%), followed by Penicillium, Mucor, Rhizopus and Fusarium. Mycotoxicological analyses did not show the presence of ochratoxins, but the presence of aflatoxins was observed in 33.75% (27/80) of the samples, with contamination levels between 1 and 3 μg/kg. |
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