Influence of lamb rennet paste on composition and proteolysis during ripening of Pecorino foggiano cheese |
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Affiliation: | 1. Faculty of BioSciences and Technology for Food, Agriculture and Environment, University of Teramo, Via C.R. Lerici 1, 64023 Mosciano Sant'' Angelo, Italy;2. Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Ave. Agustín Escardino 7, 46980 Paterna, Valencia, Spain;3. Department of Genetics, Universitat de València, C/Dr. Moliner, 56100 Burjassot, Valencia, Spain;2. Reference Center for Lactobacilli (CERELA-CONICET), San Tucumán, Argentina;3. School of Food and Nutritional Sciences, University College, Cork, Ireland;4. University of Limerick, Limerick, Ireland;5. INRA, Agro-Paris Tech, Paris, France |
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Abstract: | Rennet pastes produced by lambs subjected to three different feeding systems (mother suckling MS], artificial rearing AR], and artificial rearing with Lactobacillus acidophilus supplementation ARLB]) and slaughtered at two different ages (20 and 40 d) were used for the manufacture of Pecorino foggiano cheese. Composition and proteolysis during ripening of Pecorino foggiano cheese (four replicates batches) were analyzed. Proteolysis was greater in cheeses made with rennet pastes from lambs slaughtered at 20 d, as shown by analysis of nitrogen fractions (water-soluble N and proteose peptones). Supplementation of milk substitute with L. acidophilus may have influenced the growth dynamics of lactic acid bacteria in the rennet pastes, with positive effects on levels of lactobacilli in cheese at the beginning of the ripening time. Lower pH values in ARLB cheese during ripening, together with higher cell loads, suggest that supplementation of milk replacer with L. acidophilus resulted in higher proteolytic activity, as also confirmed by the composition of the pH 4.6—insoluble nitrogen fraction. No differences were found in total concentration of free amino acids among the experimental cheeses; phenylalanine, isoleucine, leucine, lysine were found at the highest levels. The addition of probiotic bacteria to milk substitute in lamb rearing appears to give good-quality lamb rennet paste. |
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