Application of an indirect ELISA and a PCR technique for detection of cows’ milk in sheep's and goats’ milk cheeses |
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| Affiliation: | 1. Department of Health Science, Magna Græcia University of Catanzaro, Catanzaro 88100, Italy;2. Interdepartmental Services Centre of Veterinary for Human and Animal Health, Magna Græcia University of Catanzaro, Catanzaro 88100, Italy;3. Department of Clinical and Experimental Medicine, Magna Græcia University of Catanzaro, Catanzaro 88100, Italy;1. Xilin Gol Institute of Bioengineering, Xilin Gol Food Testing and Risk Assessment Centre, Xilingol Vocational College, China;2. Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, China;1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China;2. Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China;1. Hubei Collaborative Innovation Center for Industrial Fermentation, College of Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei 430068, PR China;2. Key Laboratory of Biomarker Based Rapid-Detection Technology for Food Safety of Henan Province, Food and Biological Engineering College, Xuchang University, Xuchang 461000, PR China;3. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, PR China |
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| Abstract: | We have applied a polymerase chain reaction (PCR) procedure and an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the presence of cows’ milk in sheeps’ and goats’ milk cheeses. The PCR used a cattle-specific primer set targeting a 223 bp fragment from the mitochondrial 12S rRNA gene. This technique was applied to experimental cheese mixtures, industrial cheeses produced with known amounts of cow's milk, and several commercial cheeses, enabling the detection of low percentages of cows’ milk (1%). An indirect ELISA using a monoclonal antibody (AH4) against bovine β-casein was also assayed in this study for the specific detection of bovine milk in cheese. Results suggested that both ELISA and PCR may provide specific and reliable tools for detection of low percentages of undeclared cows’ milk in sheeps’ and/or goats’ milk cheeses and other dairy products. |
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