Concurrent denitrification and oxygen uptake in microbial films

Mixed microbial films were grown on the inner walls of a tubular reactor with recirculation of the reactor contents and continuous flow-through of nutrient solution. The loss of total oxidized nitrogen was correlated to the film population, the nitrite concentration and the dissolved oxygen concentration in the reactor. When film population was greater than 0.5 × 10⁹ cells cm⁻², reactor dissolved oxygen concentration greater than 1 mg l⁻¹ had little effect on the nitrate loss. Nitrate loss declined for film populations greater than 2 × 10⁹ cells cm⁻². Models based on Monod and zero-order microbial kinetics were calibrated using these data and a nonlinear least squares method. There was little difference in the residual errors with these methods.