| 酵母葡聚糖的制备及其理化性质研究 |
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| 引用本文： | 黄国宏,李科德,曾庆孝.酵母葡聚糖的制备及其理化性质研究[J].食品与发酵工业,2007,33(8):108-112. |
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| 作者姓名： | 黄国宏 李科德 曾庆孝 |
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| 作者单位： | 1. 华南理工大学轻工与食品学院,广东广州,510640;广西职业技术学院,广西南宁,530226
2. 华南理工大学轻工与食品学院,广东广州,510640 |
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| 摘 要： | 采用酶-碱法从酵母自溶残渣中提取β-1,3-D-葡聚糖,研究了提取工艺,并通过正交试验得出理想的酶处理工艺条件为:酶添加量为1600 IU/g,酶解3 h,pH8,温度60℃。碱处理工艺条件为:用60 mL 2%NaOH溶液在75℃处理酶解后的沉淀物6 h。冷冻干燥后得到成品葡聚糖,成品得率21.38%,其中多糖含量为92.17%,蛋白质含量为1.32%,水分含量为5.53%,该工艺具有得率高、蛋白质含量低的特点。应用红外光谱对糖分进行分析,成品为高纯度的酵母葡聚糖。
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| 关 键 词： | 酵母细胞壁 葡聚糖 酶-碱法 提取 |
| 修稿时间： | 2007-01-29 |
Extraction and Physical-chemical Properties of Glucan from Saccharomyces cervisiae |
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| Huang Guohong,Li Kede,Zeng Qingxiao.Extraction and Physical-chemical Properties of Glucan from Saccharomyces cervisiae[J].Food and Fermentation Industries,2007,33(8):108-112. |
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| Authors: | Huang Guohong Li Kede Zeng Qingxiao |
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| Affiliation: | College of Light Industry and Food Sciences,South China Univ. of Tech. ,Guangzhou 510640, 2(Guangxi Vocational Technic College,Nanning 530226, China |
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| Abstract: | The technology for extracting theβ-1,3-glucan from Saccharomyces cerevisiae by enzyme-alkali method was studied in this paper.We added 1600IU per gram yeast cell wall of alcalase and incubated for 3 hours at pH 8 and 60℃,then the precipitate was scattered with 60 mL 2% NaOH and water-bathed 6 hours at 75℃.The glucan was dried by the vacuum freeze-drying.We found that the yield of the final product was 21.38% and the content of polysaecharide,protein and moisture were 92.17% ,1.32% ,5.53%,respectively. The polysaceharide components were analyzed by Infrared spectra.The results indicated that the product ofβ- 1,3-gluean has high-purity. |
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| Keywords: | Saccharomyces cervisiae glucan enzyme-alkali method extraction |
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