虎杖中抗氧化成分的提取分离及其活性研究

提取了虎杖中具有抗氧化活性的总蒽醌(TA),并从总蒽醌中依次分离出强酸成分(SAP)、中酸成分(MAP)和弱酸成分(WAP)。然后采用二苯代苦味酰自由基(DPPH)法对不同质量浓度的TA、SAP、MAP和WAP进行了抗氧化活性测定。TA、SAP、MAP和WAP质量浓度分别为0.2 g/L、0.5 g/L、0.8 g/L、1.2 g/L时,对自由基的最大清除率依次分别为:29.5%、67.1%、85.7%、87.5%;34.0%、74.2%、86.5%、95.4%;9.8%、24.0%、35.2%、47.3%;6.5%、11.1%、19.6%、19.9%。TA、SAP、MAP和WAP对自由基均有程度不同的清除作用,其中SAP的效果最佳,当其质量浓度为1.2 g/L时,最大清除率可高达95.4%。

Separation and Activity of Antioxidant Components from Polygonum Cuspidatum

The total anthraquinones(TA) were extracted from Polygonum cuspidatum,and the strong acid part(SAP),middle acid part(MAP) and weak acid part(WAP) were separated sequencely from the total anthraquinones.Then their antioxidant activities were measured with 1,1-diphenyl-2-picrylhydrazyl(DPPH) method.When the mass concentrations were 0.2 g/L,0.5 g/L,0.8 g/L and 1.2 g/L,the highest free radical scavenging rates of TA were 29.5%,67.1%,85.7%and 87.5%,of SAP were 34.0%,74.2%,86.5% and 95.4%,of MAP were 9.8%,24.0%,35.2% and 47.3%,and of WAP were 6.5%,11.1%,19.6% and 19.9% respectively.Hence,TA,SAP,MAP and WAP showed different free radical scavenging activity.SAP was the best,and the highest free radical scavenging rate was 95.4% when mass concentration of SAP was 1.2 g/L.