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Production of fructose-1, 6-diphosphate (FDP) by permeabilized saccharomyces cerevisiae and a kinetic model for FDP accumulation
引用本文:宋达,徐志南,岑沛霖,颜学峰. Production of fructose-1, 6-diphosphate (FDP) by permeabilized saccharomyces cerevisiae and a kinetic model for FDP accumulation[J]. 中国化学工程学报, 1997, 5(3): 219-225
作者姓名:宋达  徐志南  岑沛霖  颜学峰
作者单位:Department of Chemical Engineering,Zhejiang University,Department of Chemical Engineering,Zhejiang University,Department of Chemical Engineering,Zhejiang University,Department of Chemical Engineering,Zhejiang University Hangzhou 310027,China,Hangzhou 310027,China,Hangzhou 310027,China,Hangzhou 310027,China
基金项目:Supported by the National Natural Science Foundation of China
摘    要:Permeable yeast cells were used in the batch production of fructose-1,6-diphosphate(FDP).The optimum reaction conditions were reported to be:reaction temperature 30℃,tolueneconcentration 8%(V/V),and initial ratio of glucose to inorganic phosphorus(Pi)10:1.Addition ofAMP was found to be very beneficial to the FDP production.A multienzyme system model for FDPaccumulation was developed,in which FDP was regarded as a substrate of phosphor-fructokinase(PFK),to simulate the activation effect of FDP on PFK.The model simulations were in good agree-ment with the experimental data.

关 键 词:fructose-1  6-diphosphate  Saccharomyces cerevisiae  enzyme reaction kinetics
收稿时间:1996-06-18
修稿时间: 

Production of fructose-1, 6-diphosphate (FDP) by permeabilized saccharomyces cerevisiae and a kinetic model for FDP accumulation
SONG Da,XU Zhinan,CenPeilin,YAN Xuefeng. Production of fructose-1, 6-diphosphate (FDP) by permeabilized saccharomyces cerevisiae and a kinetic model for FDP accumulation[J]. Chinese Journal of Chemical Engineering, 1997, 5(3): 219-225
Authors:SONG Da  XU Zhinan  CenPeilin  YAN Xuefeng
Affiliation:Department of Chemical Engineering, Zhejiang University, Hangzhou 310027, China
Abstract:
Keywords:fructose-1  6-diphosphate   Saccharomyces cerevisiae   enzyme reaction kinetics
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