| 电镜超薄切片批量染色的载网固定装置改进与应用 |
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| 引用本文： | 林方兴,范晓燕,沈亚峰,胡适,杨勇骥,雷长海.电镜超薄切片批量染色的载网固定装置改进与应用[J].电子显微学报,2017,36(1). |
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| 作者姓名： | 林方兴 范晓燕 沈亚峰 胡适 杨勇骥 雷长海 |
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| 作者单位： | 上海第二军医大学生物物理学教研室,上海,200433 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 电镜图像对生物组织、细胞形态结构的研究具有重要作用,高质量的超薄切片染色在透射电镜样本制备中起着关键作用.本文介绍了针对目前用于批量染色的载网固定方式存在的超薄切片甚至载网脱落等问题,对载网固定装置和技术进行了改进,从而明显地提高了批量染色的工作质量和效率.
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| 关 键 词： | 超薄切片 批量染色 装置 |
Improvement and application of grids fixing equipment for TEM ultrathin section batch staining |
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| LIN Fang-xing,FAN Xiao-yan,SHEN Ya-feng,HU Shi,YANG Yong-ji,LEI Chang-hai.Improvement and application of grids fixing equipment for TEM ultrathin section batch staining[J].Journal of Chinese Electron Microscopy Society,2017,36(1). |
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| Authors: | LIN Fang-xing FAN Xiao-yan SHEN Ya-feng HU Shi YANG Yong-ji LEI Chang-hai |
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| Abstract: | Transmission electron microscope (TEM) image plays an important role in the researches of biological tissue and cell morphology.High quality ultrathin slice staining is the key factor in TEM sample preparation.Aiming at the problems in ultrathin section batch staining work,a new type of grids fixing equipment depicted in this paper is developed to overcome the inconvenience of the traditional instrument.It can improve the quality and efficiency of batch staining. |
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| Keywords: | ultrathin section batch staining equipment |
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