Characterization of DNA hybridization kinetics in a microfluidic flow channel |
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| Affiliation: | 1. Department of Material Science and Engineering, University of California, Irvine, CA 92697, USA;2. Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA;3. Department of Medicine/Infectious Diseases, University of California, Irvine, CA 92697, USA |
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| Abstract: | In this investigation we report on the influence of volumetric flow rate, flow velocity, complementary DNA concentration, height of a microfluidic flow channel and time on DNA hybridization kinetics. A syringe pump was used to drive Cy3-labeled target DNA through a polydimethylsiloxane (PDMS) microfluidic flow channel to hybridize with immobilized DNA from the West Nile Virus. We demonstrate that a reduction of channel height, while keeping a fixed volumetric flow rate or a fixed flow velocity, enhances mass transport of target DNA to the capture probes. Compared to a passive hybridization, the DNA hybridization in the microfluidic flow channel generates higher fluorescence intensities for lower concentration of target DNA during the same fixed period of time. Within a fixed 2 min time period the fastest DNA hybridization at a 50 pM concentration of target DNA is achieved with a continuous flow of target DNA at the highest flow rate and the lowest channel height. |
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