Improvement in the measurement of spectrophotometric data in the m-hydroxydiphenyl pectin determination methods |
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| Affiliation: | 1. Grupo de Materiais Poliméricos e Compósitos (GMPC), Av. Colombo, 5790, 87020-900 Maringá, Paraná, Brazil;2. Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 86400-000 Dois Vizinhos, Paraná, Brazil;3. Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil;1. Laboratoire Analyse, Valorisation et Sécurité des Aliments, Université de Sfax, Ecole Nationale d’Ingénieurs de Sfax (ENIS), BP 1173-3038 Sfax, Tunisia;2. UMR408 SQPOV Sécurité et Qualité des Produits d’Origine Végétale, INRA, Avignon Université, F-84000 Avignon, France;3. UMR Ingénierie Procédés Aliments, AgroParisTech, INRA, Université Paris-Saclay, F-91300 Massy, France;1. Non-Destructive Biomedical and Pharmaceutical Research Centre, iPROMISE, Universiti Teknologi MARA, Puncak Alam, 42300, Selangor, Malaysia;2. Particle Design Research Group, Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam, 42300, Selangor, Malaysia;3. Malaysian Cocoa Board, Cocoa Innovative and Technology Centre, Lot 12610, Kawasan Perindustrian Nilai, Nilai, 71800, Negeri Sembilan, Malaysia;4. Razak School of Engineering and Advanced Technology, Universiti Teknologi Malaysia, 54100, Kuala Lumpur, Malaysia;1. Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia;2. Department of Process Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia;3. NMR Centre, Ruđer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia;4. Department of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia |
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| Abstract: | In the pectin analysis of foods by visible spectrophotometry using the m-hydroxydiphenyl (MHDP) as the chromogenic reagent, the evolution of the absorbance at 520 nm of the MHDP/5FF chromophore with respect to the elapsed time from the beginning of the reaction for different d-galacturonic acid concentrations was studied.It was observed that after 45 min, the stability of the readings predicted by the different methods was not reached. This evolution depended on the d-galacturonic acid concentration. The need to analyse the samples in sequence was noted, in such a way that after the addition of MHDP, a protocol was established so that the spectrophotometric data measurements taken at the highest absorbance have high reproducibility. |
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