Biomimetic Mg- and Mg,CO3-substituted hydroxyapatites: synthesis characterization and in vitro behaviour |
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| Affiliation: | 1. ISTEC-CNR, Institute of Science and Technology for Ceramics—National Research Council, via Granarolo 64, 48018 Faenza (RA), Italy;2. Istituto di Morfologia Umana Normale, Università Politecnica delle Marche, via Tronto 10/A, 60020 Ancona, Italy;3. Dipartimento di Patologia Molecolare e Terapie Innovative, Università Politecnica delle Marche, via Tronto 10/A, 60020 Ancona, Italy;1. China University of Mining and Technology, Xuzhou, 221116, PR China;2. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia;3. Hebei University of Technology, Tianjin, 300130, PR China;1. Laboratoire des Matériaux Céramiques et Procédés Associés (Université de Valenciennes et du Hainaut Cambresis), Bd. Charles de Gaulle, 59600 Maubeuge, France;2. Belgian Ceramic Research Center, Av. du Gouverneur E. Cornez 4, 7000 Mons, Belgium;1. Chemistry of Department, School of Science, Tianjin University, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, China;2. Stomatological Hospital, Tianjin Medical University, Tianjin, China;1. Biomaterials research laboratory, Faculty of Materials Science and Applied Chemistry, Riga Technical University, 3/7 Paula Valdena Street, Riga LV-1048, Latvia;2. Department of Chemistry in Pharmaceutical Science, Faculty of Pharmacy, Complutense University, Plaza de Ramón y Cajal, Ciudad Universitaria, Madrid 28040, Spain |
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| Abstract: | The doping of the apatite with carbonate or/and Mg ions in biologically-like amounts (6 and 1 wt.%, respectively) was performed. Chemico-physical characterizations and cell culture tests were carried out onto the synthetic Mg- and Mg,CO3-substituted (～30–40 nm particle size) powders in comparison with stoichiometric HA (～160 nm particle size) to determine as mesenchymal stem cells (MSCs) can directly use the mineral microenvironment to stimulate their own proliferation and differentiation activities. At the same time the growth of human osteoblast like cells (MG-63) was evaluated to determine the compatibility of the synthetic doped apatites for bone substitution. Cell morphology analysis by SEM as well as MTT and ALP tests were performed.The peculiar chemico-physical properties of the doped (Mg- and Mg,CO3-substituted) materials improved the behaviours of MSC and MG-63 cells in term of adhesion, proliferation and metabolic activation compared to stoichiometric HA. |
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