Electrochemical characteristics of heme proteins in hydroxyethylcellulose film |
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| Affiliation: | 1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China;2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China;3. Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China;4. Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, PR China;1. Department of Materials Science & Engineering and Samtel Center for Display, Technologies, Indian Institute of Technology Kanpur, Kanpur, 208016, India;2. Department of Biological Sciences & Bioengineering, Indian Institute of Technology, Kanpur, Kanpur, 208016, India;3. Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570020, India;1. Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, Bratislava SK-812 37, Slovak Republic;2. Institute of Chemistry, Department of Analytical Chemistry, Karl-Franzens University, Universitätsplatz 1, Graz A-8010, Austria;1. Flexible Information Device Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 34129, Republic of Korea;2. Research Institute of Electrical Communication, Tohoku University, Sendai, Miyagi 980-8577, Japan |
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| Abstract: | A stable film made from hydroxyethylcellulose (HEC) on pyrolytic graphite (PG) electrode was employed for incorporating hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP), and the electrochemical characteristics of the proteins were studied correspondingly. Experimental results revealed that HEC film could greatly accelerate electron transfer between the proteins and electrode, and the proteins showed a thin layer electrochemical behavior in the film. Moreover, all the proteins in the film exhibited good catalytic activity towards the reduction of hydrogen peroxide (H2O2) in the low H2O2 concentration range. In the high concentration range, H2O2 would exhibit toxicity effect on the proteins. The electrochemical properties and electrocatalytic abilities of the three heme proteins in HEC film have been compared, and the optimal conditions for H2O2 biosensor fabrication have been obtained. |
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