Effect of monodispersed silica nanoparticles on DNA separation by micro-capillary electrophoresis |
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| Affiliation: | 1. Innovative Joint Research Center, Shizuoka University, 3-5-1 Johoku Hamamatsu 432-8561, Japan;2. Department of Material Science, Graduate School of Engineering, Shizuoka University, 3-5-1 Johoku Hamamatsu 432-8561, Japan;3. Graduate School of Electronic Science and Technology, Shizuoka University, 3-5-1 Johoku Hamamatsu 432-8561, Japan;4. Department of Material Research Science, Kitami Institute of Technology, 165 Kouen-cho Kitami 090-8507, Japan |
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| Abstract: | In this work, we investigate micro-capillary electrophoresis (μ-CE) using monodispersed SiO2 nanoparticles added to an electrophoresis buffer solution to obtain high mobility and high separation of double-strand DNA. Various particle sizes of monodispersed SiO2 nanoparticle solutions were mixed with conventional 0.7% hydroxyl propyl methyl cellulose (HPMC) buffer solution to achieve μ-CE. We achieved perfect separation of the DNA specimen (100 bp to 1.5 kbp) at a certain SiO2 nanoparticle size. In addition, we discuss the effect of SiO2 nanoparticles during electrophoresis. |
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