Membrane based detection of genetically modified organisms in some representatives food |
| |
| Affiliation: | 1. Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia;2. Department of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia;3. Biotechnology Research Institute, University Malaysia Sabah, 88999 Kota Kinabalu, Sabah, Malaysia;1. College of Life Science, Northeast Agricultural University, Changjiang Road 600, Harbin 150030, China;2. College of Animal Science, Yangtze University, Nanhuan Road 1, Jingzhou 434020, China;1. Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain;2. Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain;1. Institute of Food Safety & Analytical Science, Nestec Ltd., Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland;2. Nestlé Quality Assurance Center, Quality Road, Singapore |
| |
| Abstract: | Recently, DNA-based techniques became very common for the detection of genetically modified organisms (GMOs) in food products. For rapid and easy detection of GMOs, polymerase chain reaction (PCR) screening methods, which amplify common transgenic elements, are applied in routine analysis. Incorporation of PCR and membrane method introduced in this study offer an alternative detection of GMOs. In this study, a total of 32 samples and three certified reference materials were tested for the existence of the 35S promoter of cauliflower mosaic virus (CaMV) and 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene residues. Dot blot screening system introduced in this study can be routinely used as a semi-quantitative screening of GMOs. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
