| 紫杉醇抑制原代培养人视网膜色素上皮细胞增殖的实验研究 |
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| 引用本文： | 郭惠玲,吴雅臻,黄一飞. 紫杉醇抑制原代培养人视网膜色素上皮细胞增殖的实验研究[J]. 压电与声光, 2010, 0(4) |
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| 作者姓名： | 郭惠玲 吴雅臻 黄一飞 |
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| 作者单位： | 解放军总医院;吉林大学第二医院眼科; |
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| 基金项目： | 国家自然科学基金资助项目，国家重点基础研究发展计划基金资助项目? |
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| 摘 要： | 目的研究紫杉醇(Taxol)对体外原代培养人视网膜色素上皮(human Retinal Pigment Epithelium,hRPE)细胞增殖的抑制作用及机制。方法体外分离、原代培养hRPE细胞,采用免疫细胞化学方法对其进行鉴定。不同浓度紫杉醇(0、0.005、0.05、0.5、5mg/L)处理hRPE细胞一定时间,采用形态学观察、细胞生长曲线及MTT法检测药物对hRPE细胞的生长抑制效应,流式细胞术检测药物对hRPE细胞的细胞周期阻滞作用及凋亡诱导作用。结果原代培养的hRPE细胞胞浆富含色素,随传代次数增加,黑色素颗粒逐渐减少直至消失。用抗人细胞角蛋白抗体进行免疫细胞化学鉴定hRPE细胞呈特异的阳性反应。细胞生长曲线及MTT法显示:紫杉醇作用于hRPE细胞24h和72h,其抑制细胞生长增殖的IC50值分别为5.24和3.24mg/L。流式细胞分析术检测结果显示:0.5mg/L紫衫醇作用细胞48h即可显著延迟hRPE细胞G2/M期进展并诱导凋亡(P<0.05)。透射电镜观察显示:0.5mg/L紫杉醇作用后细胞表面微绒毛减少,电子密度增加,细胞器减少,异染色质聚集成团、边聚等。结论紫杉醇通过阻滞G2/M期进展...
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| 关 键 词： | 玻璃体视网膜病变 增生性 色素上皮 眼 细胞培养技术 紫杉醇 |
Taxol inhibits proliferation of primarily cultured human retinal pigment epithelial cells |
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| GUO Hui-ling,WU Ya-zhen,HUANG Yi-fei. Taxol inhibits proliferation of primarily cultured human retinal pigment epithelial cells[J]. Piezoelectrics & Acoustooptics, 2010, 0(4) |
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| Authors: | GUO Hui-ling WU Ya-zhen HUANG Yi-fei |
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| Affiliation: | GUO Hui-ling1,WU Ya-zhen2,HUANG Yi-fei1 1Department of Ophthalmology,Chinese PLA General Hospital,Beijing 100853,China,2Department of Ophthalmology,Second Affiliated Hospital of Jilin University,Changchun 130041 |
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| Abstract: | Objective To study the inhibitory effect of Taxol on proliferation of primarily cultured in vitro human retinal pigment epithelial(hRPE) cells and its mechanism. Methods After in vitro isolation and primary culture, hRPE cells were identified with immunocytochemistry method. Inhibitory effects of Taxol at different concentrations(0, 0.005, 0.05, 0.5, and 5mg/L) on growth of hRPE cells were observed by morphology, growth curve and MTT assay, respectively, while its cell cyclearresting and apoptosis-inducing ... |
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| Keywords: | Vitreoretinopathy Proliferative Pigment Epithelium of Eye Cell Culture Techniques Taxol |
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